Pilot Gene

Pilot 1200 Digital PCR System

Pilot 1200 Digital PCR System

Chip-based digital PCR system with 20,000 partitions and 4-color detection for absolute quantification.

APPLICATIONS

Chip-based dPCR with 20,000 partitions for absolute quantification.

From rare mutation detection to 4-plex CNV in a compact system.

Absolute Quantification without Standards

Microfluidic chip dengan 20,000 partitions ~0.8 nL memungkinkan kuantifikasi absolut copy number per µL tanpa standard curve. Ideal untuk low-abundance targets dan sample volume terbatas.

Rare Mutation & ctDNA Detection

Partisi 20,000 chamber memisahkan template DNA individual untuk deteksi mutasi langka di background wild-type tinggi. Cocok untuk liquid biopsy, tumor monitoring, dan residual disease analysis.

4-Plex Multiplex CNV Analysis

4 detection channels FAM, VIC/HEX, ROX, Cy5 mendukung multiplexing hingga 4 target per well. Akurasi tinggi untuk copy number variation, gene therapy validation, dan allelic discrimination.

Viral Load Quantification

Kuantifikasi absolut RNA/DNA virus seperti HBV, HIV, HPV tanpa kalibrasi kurva. Chip-based system meminimalkan kontaminasi dan cocok untuk clinical research sample volume mikro.

Gene Expression & miRNA Analysis

Compatible dengan Pilot dPCR EvaGreen dan Probe Master Mix untuk kuantifikasi absolut mRNA dan miRNA ekspresi rendah. High partition number meningkatkan presisi untuk target ekspresi rendah.

Compact Lab-Friendly dPCR

Chip-based design + integrated thermal cycling membuat Pilot 1200 ideal untuk lab dengan space terbatas. Throughput 32 sampel per run dengan workflow sederhana dan software analysis intuitive.

Overview

Pilot 1200 Digital PCR System pakai microfluidic chip technology untuk mempartisi tiap sample jadi 20,000 chambers volume ~0.8 nL. Dilengkapi integrated thermal cycling dan 4-channel fluorescence detection untuk absolute quantification DNA/RNA tanpa standard curves. Sample capacity 8 sampel per chip, 4 chip per run. Compatible dengan probe-based dan EvaGreen assays untuk rare mutation, CNV, dan viral load quantification.

Specifications

System Type Microfluidic chip-based digital PCR
Sample Capacity 8 samples per chip, 4 chips per run
Partitions per Sample 20,000
Partition Volume ~0.8 nL
Detection Channels 4 channels: FAM, VIC/HEX, ROX, Cy5
Multiplexing Up to 4 targets per well
Excitation Source LED
Detection CMOS camera
Thermal Cycler Integrated
Temperature Range 4°C to 99°C
Dynamic Range 5 orders of magnitude
Sensitivity Detects 1 copy per reaction
Run Time ~2.5 hours for 32 samples
Hands-on Time <15 minutes
Software Pilot Analysis Software
Data Export CSV, PDF
Dimensions 48 x 42 x 35 cm (W x D x H)
Weight 28 kg
Downstream Molecular Validation

Molecular Readout
Systems

Luminescence, fluorescence, and post-PCR molecular interpretation for integrated amplification workflows.

Explore GloMax
Promega GloMax Detection Systems

Complete Your Workflow

Optimized consumables and reagents for Pilot 1200 Digital PCR System

Pilot 1200 Chips

Pilot 1200 dPCR Chip 20K

SKU: PG-CHIP-20K

Chip mikrofluidik berpresisi tinggi untuk membagi satu sampel menjadi 20.000 partisi ruang nanoliter secara otomatis dan homogen.

Partitions: 20,000 chambers
Throughput: 8 samples/chip
Material: Optical Quartz Glass
Pilot 1200 Chips

Pilot 1200 Chip Sealing Film

SKU: PG-SEAL-FLM

Lapisan film khusus berkekuatan rekat tinggi untuk menyegel chip dPCR, mencegah penguapan dan kontaminasi silang antar partisi.

Type: Pressure-sensitive Film
Clarity: High Optical Transparency
Application: Chip Sealing
dPCR Master Mixes

Pilot dPCR Probe Master Mix

SKU: PG-MMIX-PRB

Master mix teroptimasi untuk stabilitas partisi tinggi, menghasilkan pemisahan kluster positif-negatif yang kontras pada assay probe.

Concentration: 2X Mix
Application: Probe-based dPCR
Compatibility: FAM/VIC/HEX/ROX/Cy5
dPCR Master Mixes

Pilot dPCR EvaGreen Master Mix

SKU: PG-MMIX-EG

Formulasi master mix siap pakai berbasis pewarna interkalasi EvaGreen, meminimalkan inhibisi PCR di dalam ruang mikropartisi.

Concentration: 2X Mix
Dye Tech: EvaGreen Dye
Application: Absolute Quantification

Precision is Language. Integrity is Law