Illumina

NextSeq 2000

NextSeq 2000

Scalable benchtop sequencer with P1/P2/P3 flow cells, 100Gb–1.2Tb output for diverse genomics applications.

APPLICATIONS

Scalable benchtop NGS with 1.2Tb output and DRAGEN analysis.

From P1 targeted panels to P3 whole genomes with XLEAP-SBS accuracy.

Scalable Throughput with P1/P2/P3 Flow Cells

Pilih P1 10–60Gb untuk panel kecil/QC, P2 40–240Gb untuk WES rutin, atau P3 120–1200Gb untuk deep WGS dan oncology. Dual flow cells independent runs memungkinkan 2 project berbeda jalan bersamaan.

Whole Exome & Whole Genome Sequencing

P3 flow cell output 1.2Tb + PE150 ~44h memungkinkan WES 100x coverage untuk 6–8 exomes atau WGS 30x untuk 1–2 genomes per run. Q30 ≥80% dan >90% bases >Q30 menjamin data quality tinggi.

Comprehensive Oncology & CGP

Compatible dengan TruSight Oncology 500 untuk analisis 523 gen + TMB + MSI + fusion genes. XLEAP-SBS chemistry meningkatkan akurasi variant calling untuk somatic mutation dan tumor profiling klinis.

RNA-Seq & Transcriptomics

Read length PE150/PE300 + P2/P3 throughput ideal untuk gene expression profiling, isoform detection, dan fusion transcript analysis. Illumina RNA Prep with Enrichment mendukung targeted transcriptomics.

Targeted Panels & Liquid Biopsy

P1 flow cell PE150 ~13h dengan output 10–60Gb cocok untuk targeted cancer panels, amplicon sequencing, dan ctDNA analysis. Sample input rendah 1–500 ng optimal untuk FFPE dan plasma DNA.

Rapid Data Analysis with DRAGEN

On-board DRAGEN Bio-IT Platform + BaseSpace Sequence Hub memberikan push-button secondary analysis: alignment, variant calling, dan annotation. Wi-Fi + Ethernet untuk cloud integration dan remote monitoring.

Overview

NextSeq 2000 delivers flexible throughput dan simple operation untuk production-scale genomics. Memiliki XLEAP-SBS chemistry, dual flow cell architecture independent runs, dan tiga tipe flow cell P1/P2/P3 untuk output 10Gb sampai 1.2Tb. Integrated DRAGEN Bio-IT Platform provides push-button secondary analysis. Sample input 1–500 ng. Ideal untuk exomes, transcriptomes, targeted panels, small whole genomes, dan oncology CGP.

Specifications

Sequencing Technology Sequencing by Synthesis - XLEAP-SBS
Flow Cell Type P1, P2, P3 - dual flow cell, independent runs
Max Reads per Run P1: 100M, P2: 400M, P3: 1.2B reads
Read Length SE50, SE100, PE50, PE100, PE150, PE300
Output per Run P1: 10–60Gb, P2: 40–240Gb, P3: 120–1200Gb
Run Time P1 PE150: ~13h, P2 PE150: ~24h, P3 PE150: ~44h
Quality Score Q30 ≥80%, >90% bases higher than Q30
Applications WES, WGS, RNA-Seq, targeted panels, metagenomics, oncology
Sample Input 1–500 ng DNA/RNA
Instrument Control 15.6" HD Touchscreen + Cloud
Dimensions 63.5 x 57.2 x 66.0 cm (W x D x H)
Weight 108 kg
Data Analysis On-board DRAGEN + BaseSpace Sequence Hub
Connectivity Ethernet, Wi-Fi, USB 3.0
Power Requirements 100–240 VAC, 50/60 Hz, 1200W typical

Complete Your Workflow

Optimized consumables and reagents for NextSeq 2000

Flow Cells & Reagents

NextSeq 2000 P1 Reagents (100 cycles)

SKU: 20169736

Kit reagen P1 berkapasitas paling rendah untuk analisis run berskala kecil, sangat ideal untuk sekuensing panel tertarget, RNA-Seq lokal, atau kontrol kualitas library.

Flow Cell Configuration: P1 Flow Cell
Max Output: Up to 16 Gb
Chemistry: XLEAP-SBS Chemistry
Flow Cells & Reagents

NextSeq 2000 P2 Reagents (200 cycles)

SKU: 20169741

Kit reagen kelas menengah (P2) yang fleksibel untuk memfasilitasi running rutin sampel Whole Exome Sequencing (WES) atau profil transkriptomik standar.

Flow Cell Configuration: P2 Flow Cell
Max Output: Up to 120 Gb
Chemistry: XLEAP-SBS Chemistry
Flow Cells & Reagents

NextSeq 2000 P3 Reagents (300 cycles)

SKU: 20169747

Kit cartridge kapasitas tertinggi (P3) untuk memaksimalkan hingga 1.2 Tb data, dioptimalkan bagi pengerjaan Whole Genomes skala kecil dan deep-sequencing onkologi raksasa.

Flow Cell Configuration: P3 Flow Cell
Max Output: Up to 1,200 Gb / 1.2 Tb
Chemistry: XLEAP-SBS Chemistry
Library Prep Kits

Illumina DNA Prep

SKU: 20018704

Kit preparasi pustaka DNA berbasis teknologi Bead-Linked Transposomes (BLT) yang menjamin fragmentasi genom sangat seragam secara otomatis tanpa tergantung konsentrasi input.

Technology: Bead-Linked Transposomes
Sample Input: 10–500 ng
Hands-on Time: <~3.5 hours
Library Prep Kits

Illumina RNA Prep with Enrichment

SKU: 20040533

Kit prep pustaka RNA yang dipadukan dengan pengayaan probe penangkap untuk menyaring target transkriptom spesifik, mempermudah deteksi fusi gen penginfeksi.

Application: Targeted RNA-Seq
Input Range: 10–100 ng
Target Type: Coding Exons / Coding RNA
Library Prep Kits

TruSight Oncology 500

SKU: 20040764

Assay pan-cancer komprehensif bersertifikasi untuk menganalisis 523 gen penanda tumor sekaligus, mengukur beban mutasi (TMB) dan ketidakstabilan mikrosatelit (MSI).

Application: Comprehensive Genomic Profiling (CGP)
Biomarkers: TMB, MSI, Indels, SNVs, Fusions
Target Genes: 523 Genes

Precision is Language. Integrity is Law