Bio-Rad

CFX Opus 96 Real-Time PCR System

CFX Opus 96 Real-Time PCR System
Image courtesy of Thermo Fisher Scientific

96-well real-time PCR system with 6-channel detection, uniform thermal performance, and cloud connectivity.

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Overview

The CFX Opus 96 Real-Time PCR System delivers precise, reliable results with 5-channel multiplexing plus FRET. Features uniform thermal performance with Peltier heating, shuttle optical system for consistent measurements, and cloud connectivity via BR.io or CFX Maestro Software. Includes 6 VeriFlex thermal gradient zones for fast optimization and network storage access for excellent data management.

Specifications

Sample Capacity 96-well plate
Sample Volume 1–50 μL (10–50 μL recommended)
Multiplexing Up to 5 targets per well + FRET
Excitation Source 6 filtered LEDs
Detection 6 filtered photodiodes
Excitation Range 450–684 nm
Emission Range 510–730 nm
Max Ramp Rate 5°C/sec
Average Ramp Rate 3.3°C/sec
Temperature Range 4°C to 100°C
Temperature Accuracy ±0.2°C at 90°C
Temperature Uniformity ±0.3°C well-to-well after 10 sec
Gradient Range 30–100°C
Gradient Span 1–24°C
Lid Temperature 30–110°C
Scan Time 12 sec all channels; 3 sec FAM/SYBR only
Sensitivity Detects 1 copy of target in human genomic DNA
Dynamic Range 10 orders of magnitude
Connectivity Wi-Fi, Ethernet, USB 2.0, Cloud BR.io
Dimensions 33 x 56 x 36 cm (W x D x H)
Weight 22 kg
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Luminescence, fluorescence, and post-PCR molecular interpretation for integrated amplification workflows.

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Optimized consumables and reagents for CFX Opus 96 Real-Time PCR System

Hard-Shell 96-Well PCR Plates
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Hard-Shell 96-Well PCR Plates

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Microseal B Adhesive Seals
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PCR 8-Tube Strips
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iTaq Universal SYBR Green Supermix
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iTaq Universal Probes Supermix
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Integrity is Law.

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