Roche

LightCycler 480 II

LightCycler 480 II

High-throughput 384-well qPCR platform with HRM and endpoint genotyping capabilities.

APPLICATIONS

High-throughput qPCR for genomics and clinical research.

From gene expression to HRM and multiplex pathogen detection.

High-Throughput Gene Expression Analysis

384-well block + 6 detection channels mendukung kuantifikasi ekspresi gen skala besar. Run time 40 min untuk 40 siklus mempercepat throughput penelitian biomarker dan drug discovery.

SNP Genotyping & Endpoint Analysis

Endpoint genotyping dengan probe hidrolisis atau hibridisasi. Akurasi suhu ±0.2°C dan uniformity ±0.3°C memastikan calling alel yang presisi untuk studi variasi genetik.

High Resolution Melting HRM Analysis

HRM resolution 0.1°C memungkinkan mutation scanning, methylation analysis, dan deteksi varian tanpa probe. Teknologi CCD camera + xenon lamp menghasilkan data melting curve presisi tinggi.

Pathogen Detection & Clinical Research

Multiplexing hingga 6-plex mendukung deteksi simultan bakteri, virus, dan patogen lain. Cocok untuk pengembangan assay diagnostic dan clinical research validation.

PCR Method Development with Gradient

Thermal gradient optimization mempercepat penentuan annealing temperature optimal. Meminimalkan trial-and-error saat setup assay baru pada block 96-well.

miRNA & Low-Copy Target Quantification

Volume reaksi 5–20 µL pada 384-well menghemat reagen untuk target ekspresi rendah seperti miRNA. Deteksi CCD + 6 filter set meningkatkan sensitivitas sinyal fluoresens.

Overview

LightCycler 480 II delivers high-throughput real-time PCR dengan opsi block 96 atau 384-well. Dilengkapi 6 detection channels, thermal gradient optimization, dan advanced HRM analysis resolusi 0.1°C untuk mutation scanning dan genotyping. Ideal untuk gene expression, SNP genotyping, dan pathogen detection di clinical research dengan run time 40 min untuk 40 siklus.

Specifications

Sample Capacity 96-well or 384-well block
Reaction Volume 5–20 μL (384-well), 10–100 μL (96-well)
Multiplexing Up to 6 targets / 6-plex
Excitation Source Xenon lamp
Detection CCD camera with 6 filter sets
Excitation Range 450–615 nm
Emission Range 500–670 nm
Max Block Ramp Rate 4.8°C/sec
Temperature Range 37°C to 99°C
Temperature Accuracy ±0.2°C
Temperature Uniformity ±0.3°C
HRM Resolution 0.1°C
Run Time 40 min for 40 cycles
Software LightCycler 480 Software
Connectivity LAN, USB
Dimensions 65 x 52 x 45 cm (W x D x H)
Weight 45 kg
Downstream Molecular Validation

Molecular Readout
Systems

Luminescence, fluorescence, and post-PCR molecular interpretation for integrated amplification workflows.

Explore GloMax
Promega GloMax Detection Systems

Complete Your Workflow

Optimized consumables and reagents for LightCycler 480 II

Plates & Seals

LightCycler 480 Multiwell Plate 96

SKU: 04729692001

Plat sumur putih yang didesain khusus untuk mengoptimalkan refleksi sinyal fluoresens dan menekan crosstalk antar sumur pada pembacaan kamera CCD.

Format: 96-Well Plate
Color: White
Material: High-precision Plastic
Plates & Seals

LightCycler 480 Multiwell Plate 384

SKU: 04729749001

Memastikan transfer panas yang sangat seragam di seluruh 384 sumur untuk volume reaksi mikro (5–20 µL) dalam aplikasi high-throughput.

Format: 384-Well Plate
Color: White
Reaction Vol: 5–20 µL
Plates & Seals

LightCycler 480 Sealing Foil

SKU: 04729757001

Perekat transparan optik dengan tingkat autofluoresens ultra-rendah untuk menjamin transmisi cahaya bebas distorsi ke detektor filter.

Type: Optical Adhesive Film
Clarity: Ultra-clear
Application: qPCR Seal
qPCR Reagents

LightCycler 480 SYBR Green I Master

SKU: 04707516001

Master mix siap pakai pasca-optimasi untuk kuantifikasi gen target tunggal dengan linearitas dinamis yang sangat luas.

Concentration: 2X Mix
Detection: SYBR Green I
Format: Hot-Start PCR ready
qPCR Reagents

LightCycler 480 Probes Master

SKU: 04707494001

Dioptimalkan khusus untuk assay berbasis probe hidrolisis (TaqMan), mendukung multiplexing hingga 6 target secara simultan.

Application: Probe-based qPCR
Multiplex: Up to 6-plex
Enzyme: Hot-start Taq DNA Polymerase
qPCR Reagents

LightCycler 480 Genotyping Master

SKU: 04707524001

Formulasi master mix performa tinggi untuk analisis variasi genetik (SNP) menggunakan probe hibridisasi maupun teknik HRM.

Application: SNP Genotyping / HRM
Stability: Enhanced enzyme stability
Component: Includes MgCl2 optimized

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